Myco Sphere breaking down 12 different ways you can inoculate agar plates - from classic plate-to-plate transfers, liquid culture, and spore work to more advanced methods like agar pins, long-term storage revival, fresh and dry tissue samples, and spawn. Each method offers unique insight into growth speed, contamination risk, and culture recovery. #northspore #spreadthespore #agar #mycology #fungi #mushrooms | North Spore
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Myco Sphere demonstrating three specific methods for inoculating grain: injecting liquid culture through a self-healing port, transferring wedges from a fully colonized agar plate, and expanding spawn with grain-to-grain transfers. He highlights proper technique like clean clothes, alcohol wipes, flame sterilization, and controlled airflow, and shows how each method works in practice and when to use them to produce clean, fast-colonizing grain spawn. Get started with a grain bag here (currently
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Contamination is a constant balancing act in mushroom cultivation. No matter how much experience you have, you will run into it from time to time. We have rigorous quality control here at North Spore for our sterile substrates, and even so, it can happen to us too. It’s best practice to work in a flowhood, but you can also make or buy a NocBox to maintain a clean space. Check out our full guide on the most common contaminants in mushroom cultivation, and how to avoid them 🦠: https://northspore.
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